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Proceedings Paper

Raman spectroscopic study of keratin 8 knockdown oral squamous cell carcinoma derived cells
Author(s): S. P. Singh; Hunain Alam; Crismita Dmello; Milind M. Vaidya; C. Murali Krishna
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Paper Abstract

Keratins are one of most widely used markers for oral cancers. Keratin 8 and 18 are expressed in simple epithelia and perform both mechanical and regulatory functions. Their expression are not seen in normal oral tissues but are often expressed in oral squamous cell carcinoma. Aberrant expression of keratins 8 and 18 is most common change in human oral cancer. Optical-spectroscopic methods are sensitive to biochemical changes and being projected as novel diagnostic tools for cancer diagnosis. Aim of this study was to evaluate potentials of Raman spectroscopy in detecting minor changes associated with differential level of keratin expression in tongue-cancer-derived AW13516 cells. Knockdown clones for K8 were generated and synchronized by growing under serum-free conditions. Cell pellets of three independent experiments in duplicate were used for recording Raman spectra with fiberoptic-probe coupled HE-785 Raman-instrument. A total of 123 and 96 spectra from knockdown clones and vector controls respectively in 1200-1800 cm-1 region were successfully utilized for classification using LDA. Two separate clusters with classification-efficiency of ~95% were obtained. Leave-one-out cross-validation yielded ~63% efficiency. Findings of the study demonstrate the potentials of Raman spectroscopy in detecting even subtle changes such as variations in keratin expression levels. Future studies towards identifying Raman signals from keratin in oral cells can help in precise cancer diagnosis.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251C (9 February 2012); doi: 10.1117/12.905450
Show Author Affiliations
S. P. Singh, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Hunain Alam, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Crismita Dmello, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
Milind M. Vaidya, Advanced Ctr. for Treatment, Research and Education in Cancer (India)
C. Murali Krishna, Advanced Ctr. for Treatment, Research and Education in Cancer (India)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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