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Proceedings Paper

MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging
Author(s): Qiaole Zhao; Ian Ted Young; Ben Schelen; Raymond Schouten; Rein van den Oever; René Leenen; Harry van Kuijk; Inge Peters; Frank Polderdijk; Jan Bosiers; Kees Jalink; Sander de Jong; Bert van Geest; Karel Stoop
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Paper Abstract

We have built an all-solid-state camera which is directly modulated at the pixel level for frequency domain fluorescence lifetime imaging microscopy (FLIM) measurement. This novel camera eliminates the need for an image intensifier through the use of an application-specific CCD design, which is being used in a frequency domain FLIM system. The first stage of evaluation for the camera has been carried out. Camera characteristics such as noise distribution, dark current influence, camera gain, sampling density, sensitivity, linearity of photometric response, and contrast modulation transfer function have been studied through experiments. We are able to do lifetime measurement using MEM-FLIM cameras for various objects, e.g. fluorescence plastic test slides, fluorescein solution, fixed GFP cells, and GFP - Actin stained live cells.

Paper Details

Date Published: 9 February 2012
PDF: 11 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82250N (9 February 2012); doi: 10.1117/12.905345
Show Author Affiliations
Qiaole Zhao, Technische Univ. Delft (Netherlands)
Ian Ted Young, Technische Univ. Delft (Netherlands)
Ben Schelen, Technische Univ. Delft (Netherlands)
Raymond Schouten, Technische Univ. Delft (Netherlands)
Rein van den Oever, Technische Univ. Delft (Netherlands)
René Leenen, Teledyne DALSA (Netherlands)
Harry van Kuijk, Teledyne DALSA (Netherlands)
Inge Peters, Teledyne DALSA (Netherlands)
Frank Polderdijk, Teledyne DALSA (Netherlands)
Jan Bosiers, Teledyne DALSA (Netherlands)
Kees Jalink, Netherlands Cancer Institute (Netherlands)
Sander de Jong, Lambert Instruments BV (Netherlands)
Bert van Geest, Lambert Instruments BV (Netherlands)
Karel Stoop, Lambert Instruments BV (Netherlands)

Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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