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Proceedings Paper

Probing the interior of synaptic vesicles with internalized nanoparticles
Author(s): Jennifer C. Gadd; Kristi L. Budzinski; Yang-Hsiang Chan; Fangmao Ye; Daniel T. Chiu
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Paper Abstract

Synaptic vesicles are subcellular organelles that are found in the synaptic bouton and are responsible for the propagation of signals between neurons. Synaptic vesicles undergo endo- and exocytosis with the neuronal membrane to load and release neurotransmitters. Here we discuss how we utilize this property to load nanoparticles as a means of probing the interior of synaptic vesicles. To probe the intravesicular region of synaptic vesicles, we have developed a highly sensitive pH-sensing polymer dot. We feel the robust nature of the pH-sensing polymer dot will provide insight into the dynamics of proton loading into synaptic vesicles.

Paper Details

Date Published: 2 February 2012
PDF: 9 pages
Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320A (2 February 2012); doi: 10.1117/12.905091
Show Author Affiliations
Jennifer C. Gadd, Univ. of Washington (United States)
Kristi L. Budzinski, Univ. of Washington (United States)
Yang-Hsiang Chan, Univ. of Washington (United States)
Fangmao Ye, Univ. of Washington (United States)
Daniel T. Chiu, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 8232:
Colloidal Nanocrystals for Biomedical Applications VII
Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski, Editor(s)

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