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Proceedings Paper

Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy
Author(s): Chao Liu; Yan Zhou; Xinwei Wang; Yuliang Liu
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Paper Abstract

How to simulate the decay pattern is crucial during lifetime inversion while utilizing intensity images acquired at increasing delays in time gated fluorescence lifetime imaging microscopy (FLIM) method. A relatively novel understanding of fluorescence decay pattern theory and stimulation algorithms of time gated FLIM method have been analyzed in this paper comprehensively. Main lifetime computing algorithms can be classified as exponential pattern retrieve and polynomial fitting procedure. Especially, a novel lifetime computing method based on bi-exponential decay has been discussed. In experiment, we have validated the proposed algorithms utilizing synthetic images. Performances like calculating precision and computing speed of the algorithms above have also been compared.

Paper Details

Date Published: 28 November 2011
PDF: 8 pages
Proc. SPIE 8200, 2011 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Processing Technology, 82000X (28 November 2011); doi: 10.1117/12.904352
Show Author Affiliations
Chao Liu, Institute of Semiconductors (China)
Yan Zhou, Institute of Semiconductors (China)
Xinwei Wang, Institute of Semiconductors (China)
Yuliang Liu, Institute of Semiconductors (China)


Published in SPIE Proceedings Vol. 8200:
2011 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Processing Technology
Toru Yoshizawa; Ping Wei; Jesse Zheng, Editor(s)

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