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Proceedings Paper

Line-scan focal modulation microscopy for rapid imaging of thick biological specimens
Author(s): Shau Poh Chong; Shilpa Pant; Nanguang Chen
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Paper Abstract

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal Modulation Microscopy [1-3] (FMM) is an emerging single-photon excitation fluorescence microscopy technique that can provide sub-micron spatial resolution for deep imaging of biological tissues mainly by preserving the signal-to-background ratio. Here we report a line-scan FMM that enables line-byline recording [4] at video frame rates (>30 fps) depending on the size of region of interest.

Paper Details

Date Published: 29 November 2011
PDF: 5 pages
Proc. SPIE 8311, Optical Sensors and Biophotonics III, 83111C (29 November 2011); doi: 10.1117/12.904115
Show Author Affiliations
Shau Poh Chong, National Univ. of Singapore (Singapore)
Shilpa Pant, National Univ. of Singapore (Singapore)
Nanguang Chen, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 8311:
Optical Sensors and Biophotonics III
Jürgen Popp, Editor(s)

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