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Proceedings Paper

Immobilization of enzyme (DAAO) on hybrid nanoporous MCF, SBA-15, and MCM-41 materials
Author(s): Tien Q. Phi; Hy G. Le; Tuan A. Vu; Thao T. H. Phan; Huyen T. Pham; Canh Duc Dao; Phuong T. Dang
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Paper Abstract

Hybrid nanoporous MCF, SBA-15 and MCM-41 materials were synthesized via hydrothermal treatment and functionalized with 3-aminopropyltriethoxysilane (APTES) via post-synthesis grafting and sequently activated by glutardialdehyde and then were used to immobilize D-amino acid oxidase (DAAO). The amino-functionalized materials were characterized by various techniques: XRD, IR and N2 adsorption-desorption (BET). From characterization results, it indicated that these materials still maintained their structure after functionalization. The data IR and TGA-DTA analysis demonstrated the incorpotation of amine functional groups on the surface of APTES-functionalized samples. The DAAO immobilized on functionalized materials exhibited higher catalytic activity and stability for conversion of cephalosporin C (CPC) compare to those of non-functionalized one. Further more, the catalytic activity as well as stability of enzyme decreased in order MCF > SBA-15 > MCM-41 with the decrease of their pore size.

Paper Details

Date Published: 23 December 2011
PDF: 9 pages
Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043I (23 December 2011); doi: 10.1117/12.903739
Show Author Affiliations
Tien Q. Phi, Vietnam Academy of Science and Technology (Viet Nam)
Hy G. Le, Vietnam Academy of Science and Technology (Viet Nam)
Tuan A. Vu, Vietnam Academy of Science and Technology (Viet Nam)
Thao T. H. Phan, Vietnam Academy of Science and Technology (Viet Nam)
Huyen T. Pham, Vietnam Academy of Science and Technology (Viet Nam)
Canh Duc Dao, Vietnam Academy of Science and Technology (Viet Nam)
Phuong T. Dang, Vietnam Academy of Science and Technology (Viet Nam)


Published in SPIE Proceedings Vol. 8204:
Smart Nano-Micro Materials and Devices
Saulius Juodkazis; Min Gu, Editor(s)

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