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Proceedings Paper

Evaluation of autofocus measures for microscopy images of biopsy and cytology
Author(s): R. Redondo; M. G. Bueno; J. C. Valdiviezo; R. Nava; G. Cristóbal; M. García; O. Déniz; B. Escalante-Ramírez
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Paper Abstract

An essential and indispensable component of automated microscopy is the automatic focusing system, which determines the in-focus position of a given field of view by searching for the maximal of an autofocus function over a range of z-axis positions. The autofocus function and its computation time are crucial to the accuracy and efficiency of the system. In this paper, we analyze and evaluate fifteen autofocus algorithms for biopsy and cytology microscopy images, ranging from the already well known methods to those proposed recently. Results have shown that there is a trade-off between computational cost and accuracy. Finally, the error committed by each of the algorithms is presented.

Paper Details

Date Published: 3 November 2011
PDF: 9 pages
Proc. SPIE 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World, 801194 (3 November 2011); doi: 10.1117/12.903314
Show Author Affiliations
R. Redondo, Consejo Superior de Investigaciones Científicas (Spain)
Univ. Castilla la Mancha (Spain)
M. G. Bueno, Univ. Castilla la Mancha (Spain)
J. C. Valdiviezo, Instituto Nacional de Astrofísica, Óptica y Electrónica (Mexico)
R. Nava, Univ. Nacional Autónoma de México (Mexico)
G. Cristóbal, Consejo Superior de Investigaciones Científicas (Spain)
M. García, Hospital General Ciudad Real (Spain)
O. Déniz, Univ. Castilla la Mancha (Spain)
B. Escalante-Ramírez, Univ. Nacional Autónoma de México (Mexico)


Published in SPIE Proceedings Vol. 8011:
22nd Congress of the International Commission for Optics: Light for the Development of the World
Ramón Rodríguez-Vera; Ramón Rodríguez-Vera; Ramón Rodríguez-Vera; Rufino Díaz-Uribe; Rufino Díaz-Uribe; Rufino Díaz-Uribe, Editor(s)

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