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Proceedings Paper

Integration of microplasma and microfluidic technologies for localised microchannel surface modification
Author(s): Endre J. Szili; Sameer A. Al-Bataineh; Craig Priest; Philipp J. Gruner; Paul Ruschitzka; James W. Bradley; John Ralston; David A. Steele; Robert D. Short
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Paper Abstract

In this paper we describe the spatial surface chemical modification of bonded microchannels through the integration of microplasmas into a microfluidic chip (MMC). The composite MMC comprises an array of precisely aligned electrodes surrounding the gas/fluid microchannel. Pairs of electrodes are used to locally ignite microplasmas inside the microchannel. Microplasmas, comprising geometrically confined microscopic electrically-driven gas discharges, are used to spatially functionalise the walls of the microchannels with proteins and enzymes down to scale lengths of 300 μm inside 50 μm-wide microchannels. Microchannels in poly(dimethylsiloxane) (PDMS) or glass were used in this study. Protein specifically adsorbed on to the regions inside the PDMS microchannel that were directly exposed to the microplasma. Glass microchannels required pre-functionalisation to enable the spatial patterning of protein. Firstly, the microchannel wall was functionalised with a protein adhesion layer, 3-aminopropyl-triethoxysilane (APTES), and secondly, a protein blocking agent (bovine serum albumin, BSA) was adsorbed onto APTES. The functionalised microchannel wall was then treated with an array of spatially localised microplasmas that reduced the blocking capability of the BSA in the region that had been exposed to the plasma. This enabled the functionalisation of the microchannel with an array of spatially separated protein. As an alternative we demonstrated the feasibility of depositing functional thin films inside the MMC by spatially plasma depositing acrylic acid and 1,7-octadiene within the microchannel. This new MMC technology enables the surface chemistry of microchannels to be engineered with precision, which is expected to broaden the scope of lab-on-a-chip type applications.

Paper Details

Date Published: 23 December 2011
PDF: 7 pages
Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82042J (23 December 2011); doi: 10.1117/12.903293
Show Author Affiliations
Endre J. Szili, Univ. of South Australia (Australia)
Sameer A. Al-Bataineh, Univ. of South Australia (Australia)
Craig Priest, Univ. of South Australia (Australia)
Philipp J. Gruner, Univ. of South Australia (Australia)
Paul Ruschitzka, Univ. of South Australia (Australia)
James W. Bradley, Univ. of Liverpool (United Kingdom)
John Ralston, Univ. of South Australia (Australia)
David A. Steele, Univ. of South Australia (Australia)
Robert D. Short, Univ. of South Australia (Australia)

Published in SPIE Proceedings Vol. 8204:
Smart Nano-Micro Materials and Devices
Saulius Juodkazis; Min Gu, Editor(s)

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