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Proceedings Paper

Surface modification of poly(dimethylsiloxane) (PDMS) microchannels with DNA capture-probes for potential use in microfluidic DNA analysis systems
Author(s): Dmitriy A. Khodakov; Leigh D. Thredgold; Claire E. Lenehan; Gunther A. Andersson; Hilton Kobus; Amanda V. Ellis
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Paper Abstract

Poly(dimethylsiloxane) (PDMS) is an elastomeric material used for microfluidic devices and is especially suited to medical and forensic applications. This is due to its relatively low cost, ease of fabrication, excellent optical transmission characteristics and its ability to support electroosmotic flow, required during electrophoretic separations. These aspects combined with its large range of surface modification chemistries, make PDMS an attractive substrate in microfluidic devices for, in particular, DNA separation. Here, we report the successful wet chemical surface modification of PDMS microchannels using a simple three step method to produce an isothiocyanate-terminated surface. Initially, PDMS was oxygen plasma treated to produce a silanol-terminated surface, this was then reacted with 3-aminopropyltriethoxysilane with subsequent reaction of the now amine-terminated surface with p-phenylenediisothiocyanate. Water contact angle measurements both before and after modification showed a reduction in hydrophobicity from 101o for native PDMS to 94o for the isothiocyante-terminated PDMS. The isothiocyanate-terminated surface was then coupled with an amineterminated single-stranded DNA (ssDNA) oligonucleotide capture probe via a thiourea linkage. Confirmation of capture probe attachment was observed using fluorescent microscopy after hybridization of the capture probes with fluorescently labeled complimentary ssDNA oligonucleotides.

Paper Details

Date Published: 24 December 2011
PDF: 9 pages
Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82040J (24 December 2011); doi: 10.1117/12.903208
Show Author Affiliations
Dmitriy A. Khodakov, Flinders Univ. (Australia)
Leigh D. Thredgold, Flinders Univ. (Australia)
Claire E. Lenehan, Flinders Univ. (Australia)
Gunther A. Andersson, Flinders Univ. (Australia)
Hilton Kobus, Flinders Univ. (Australia)
Amanda V. Ellis, Flinders Univ. (Australia)


Published in SPIE Proceedings Vol. 8204:
Smart Nano-Micro Materials and Devices
Saulius Juodkazis; Min Gu, Editor(s)

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