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Proceedings Paper

Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant
Author(s): Okhil Kumar Nag; Han Young Woo; Wei R. Chen
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Paper Abstract

We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N-bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

Paper Details

Date Published: 14 February 2012
PDF: 13 pages
Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240G (14 February 2012); doi: 10.1117/12.901836
Show Author Affiliations
Okhil Kumar Nag, Univ. of Central Oklahoma (United States)
Han Young Woo, Pusan National Univ. (Korea, Republic of)
Wei R. Chen, Univ. of Central Oklahoma (United States)

Published in SPIE Proceedings Vol. 8224:
Biophotonics and Immune Responses VII
Wei R. Chen, Editor(s)

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