Share Email Print
cover

Proceedings Paper

A Raman spectroscopic approach for the cultivation-free identification of microbes
Author(s): Petra Rösch; Stephan Stöckel; Susann Meisel; Ute Münchberg; Sandra Kloß; Dragana Kusic; Wilm Schumacher; Jürgen Popp
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In the last years the identification of microorganisms by means of different IR and Raman spectroscopic techniques has become quite popular. Most of the studies however apply the various vibrational spectroscopic methods to bulk samples which require at least a short cultivation time of several hours. Nevertheless, bulk identification methods achieve high classification rates which enable even the discrimination between closely related strains or the distinction between resistance capabilities. However, applying micro-Raman spectroscopy with visible excitation wavelengths enables for the detection of single microorganisms. Especially for time critical process like the fast diagnosis of severe diseases or the identification of bacterial contamination on food samples or pharmaceuticals, a cultivation-free identification of bacteria is required. In doing so, we established different isolation techniques in combination with Raman spectroscopic identification. Isolating bacteria from different matrixes always has an impact on the Raman spectroscopic identification capability. Therefore, these isolation techniques have to be specially designed to fulfill the spectroscopic requirements. In total the method should enable the identification of pathogens within the first 3 hours.

Paper Details

Date Published: 29 November 2011
PDF: 6 pages
Proc. SPIE 8311, Optical Sensors and Biophotonics III, 83111B (29 November 2011); doi: 10.1117/12.901241
Show Author Affiliations
Petra Rösch, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Stephan Stöckel, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Susann Meisel, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Ute Münchberg, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Sandra Kloß, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Dragana Kusic, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Wilm Schumacher, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Institute of Physical Chemistry, Friedrich-Schiller-Univ. Jena (Germany)
Institut für Photonische Technologien e.V. (Germany)


Published in SPIE Proceedings Vol. 8311:
Optical Sensors and Biophotonics III
Jürgen Popp, Editor(s)

© SPIE. Terms of Use
Back to Top