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Proceedings Paper

Double-kinetic time-resolved measurement of non-equilibrium fluorescence lifetime with time sequence of nanosecond-scale: a novel technique based on single photon counting
Author(s): Xiao-Chun Huang; De-Yong Li; Yu-Xiang Weng
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Paper Abstract

A novel technique based on single photon counting is developed for tracing the non-equilibrium process by recording the fluorescence lifetime changes over time. Different from the conventional time-correlated single photon counting (TCSPC) method, our technique uses a 'double-time' time-correlated strategy in the single photon counting, and it can record a sequence of fluorescence decay curves with nanosecond-scale time interval. The key point of our technique is to assign the photons to different groups according to the relative time between photon emission and the beginning of non-equilibrium process. It is achieved by a specially designed setup. In this setup, the instrument response function, contributed by the photon detector and signal processing circuits, is about 250ps. The time interval between two adjacent fluorescence decay curves is adjustable in the range of 13.5~157ns, and eight curves can be measured simultaneously. Compared with the direct waveform recording method, our novel method can provide significantly better accuracy in the measurement of fluorescence decay curves. An aqueous solution of tryptophan is used to demonstrate our method with laser induced temperature-jump technique, and the possible improvements are also discussed. The technique we introduced here has potential applications in the fields of fast biodynamic research, such as the protein folding/unfolding kinetics.

Paper Details

Date Published: 23 August 2011
PDF: 10 pages
Proc. SPIE 8192, International Symposium on Photoelectronic Detection and Imaging 2011: Laser Sensing and Imaging; and Biological and Medical Applications of Photonics Sensing and Imaging, 81924R (23 August 2011); doi: 10.1117/12.898550
Show Author Affiliations
Xiao-Chun Huang, Institute of Physics (China)
De-Yong Li, Institute of Physics (China)
Yu-Xiang Weng, Institute of Physics (China)


Published in SPIE Proceedings Vol. 8192:
International Symposium on Photoelectronic Detection and Imaging 2011: Laser Sensing and Imaging; and Biological and Medical Applications of Photonics Sensing and Imaging
Farzin Amzajerdian; Weibiao Chen; Chunqing Gao; Tianyu Xie, Editor(s)

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