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Proceedings Paper

A new optical method for a fast and simple detection of ephedrine
Author(s): Antonio Varriale; Maria Staiano; Maria Strianese; Vincenzo Marzullo; Giuseppe Ruggiero; Alberto Secchi; Massimiliano Dispenza; Anna Maria Fiorello; Sabato D'Auria
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Paper Abstract

In this work we describe the synthesis of a new ephedrine derivative with a carbon linker featuring an amino reactive group, and its conjugation to the glutamine binding protein (GlnBP) from E. coli as a carrier protein for the production of polyclonal antibodies in rabbits against ephedrine. Proof-of-principle results that an efficient SPR-based indirect competitive immunoassay for the detection and quantification of ephedrine are presented. The detection limit of this assay was found to be about 33ng/ml.

Paper Details

Date Published: 13 October 2011
PDF: 6 pages
Proc. SPIE 8189, Optics and Photonics for Counterterrorism and Crime Fighting VII; Optical Materials in Defence Systems Technology VIII; and Quantum-Physics-based Information Security, 81890M (13 October 2011); doi: 10.1117/12.898045
Show Author Affiliations
Antonio Varriale, Lab. for Molecular Sensing, CNR (Italy)
Maria Staiano, Lab. for Molecular Sensing, CNR (Italy)
Univ. of Siena (Italy)
Maria Strianese, Lab. for Molecular Sensing, CNR (Italy)
Vincenzo Marzullo, Lab. for Molecular Sensing, CNR (Italy)
Giuseppe Ruggiero, Lab. for Molecular Sensing, CNR (Italy)
Alberto Secchi, SELEX Sistemi Integrati S.p.A. (Italy)
Massimiliano Dispenza, SELEX Sistemi Integrati S.p.A. (Italy)
Anna Maria Fiorello, SELEX Sistemi Integrati S.p.A. (Italy)
Sabato D'Auria, Lab. for Molecular Sensing, CNR (Italy)


Published in SPIE Proceedings Vol. 8189:
Optics and Photonics for Counterterrorism and Crime Fighting VII; Optical Materials in Defence Systems Technology VIII; and Quantum-Physics-based Information Security
Roberto Zamboni; Mark T. Gruneisen; Colin Lewis; Miloslav Dusek; Douglas Burgess; François Kajzar; Attila A. Szep; John G. Rarity, Editor(s)

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