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Proceedings Paper

High-frequency H-PDLC optical chopper for frequency division multiplexing fluorescence confocal microscope system
Author(s): Yanmeng Jiang; Jihong Zheng; Pingyu Tang; Tingting Wang; Aiqin Huang; Zengjun Zhou; Songlin Zhuang
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Paper Abstract

The optical chopper array based on Holographic Polymer Dispersed Liquid Crystal (H-PDLC) working at high frequencies, for example 1KHz, 2KHz, and its application in an improved Frequency Division Multiplexed Fluorescence Confocal Microscope (FDMFCM) system are reported in this article. The system is a combination of the confocal microscopy and the frequency division multiplexing technique. Taking advantages of the optical chopper array based on H-PDLC that avoids mechanical movements, the FDMFCM system is able to obtain better Signal-Noise Ratio (SNR), smaller volume, more independent channels and more efficient scanning. What's more, the FDMCFM maintained the high special resolution ability and realized faster temporal resolution than pervious system. Using the proposed device, the FDMFCM system conducts successful parallel detection of rat neural cells. Fluorescence intensity signals from two different points on the specimen, which represent concentration of certain kind of proteins in the sample cells, are achieved. The experimental results show that the proposed H-PDLC optical chopper array has feasibility in FDMFCM system, which owes to its unique characteristics such as fast response, simple fabrication and lower consumption etc. With the development of H-PDLC based devices, there will be prospective in upgrading FDMFCM system's performance in the biomedical area.

Paper Details

Date Published: 8 September 2011
PDF: 9 pages
Proc. SPIE 8120, Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications V, 81201O (8 September 2011); doi: 10.1117/12.897220
Show Author Affiliations
Yanmeng Jiang, Univ. of Shanghai for Science and Technology (China)
Jihong Zheng, Univ. of Shanghai for Science and Technology (China)
Pingyu Tang, Univ. of Shanghai for Science and Technology (China)
Tingting Wang, Univ. of Shanghai for Science and Technology (China)
Aiqin Huang, Univ. of Shanghai for Science and Technology (China)
Zengjun Zhou, Univ. of Shanghai for Science and Technology (China)
Songlin Zhuang, Univ. of Shanghai for Science and Technology (China)


Published in SPIE Proceedings Vol. 8120:
Photonic Fiber and Crystal Devices: Advances in Materials and Innovations in Device Applications V
Shizhuo Yin; Ruyan Guo, Editor(s)

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