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Proceedings Paper

Detection of bacterial infection with a fiber optic microendoscope
Author(s): Nooman Mufti; Ying Kong; Jeffrey D. Cirillo; Kristen C. Maitland
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Paper Abstract

We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 106 to 101 CFU and intra-tracheal inocula of 108 to 106 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 104 CFU for skin samples and 107 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 106 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

Paper Details

Date Published: 2 June 2011
PDF: 8 pages
Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80920A (2 June 2011); doi: 10.1117/12.896553
Show Author Affiliations
Nooman Mufti, Texas A&M Univ. (United States)
Ying Kong, Texas A&M Health Science Ctr. (United States)
Jeffrey D. Cirillo, Texas A&M Health Science Ctr. (United States)
Kristen C. Maitland, Texas A&M Univ. (United States)


Published in SPIE Proceedings Vol. 8092:
Medical Laser Applications and Laser-Tissue Interactions V
Ronald Sroka; Lothar D. Lilge, Editor(s)

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