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Proceedings Paper

Nanobarcoding: a novel method of single nanoparticle detection in cells and tissues for nanomedical biodistribution studies
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Paper Abstract

Determination of whether nanoparticles accumulate in target or non-target tissues is critical in assessing a nanoparticle formulation for nanomedical purposes. There is an overwhelming need for a sensitive and efficient imaging-based method that can (1) detect small numbers of (even single) nanoparticles, (2) associate nanoparticle uptake with cell type, and (3) allow for rapid detection in large tissue samples. We propose a novel method for nanoparticle detection that utilizes an oligonucleotide "nanobarcode" conjugated to the nanoparticle surface, which amplifies the optical signal from a single nanoparticle via in situ PCR. Herein, we describe the design process of the nanobarcoding method.

Paper Details

Date Published: 12 September 2011
PDF: 13 pages
Proc. SPIE 8099, Biosensing and Nanomedicine IV, 80990V (12 September 2011); doi: 10.1117/12.893915
Show Author Affiliations
Trisha Eustaquio, Purdue Univ. (United States)
James F. Leary, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 8099:
Biosensing and Nanomedicine IV
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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