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Proceedings Paper

Characterization of microorganisms using Raman tweezers
Author(s): Ota Samek; Zdenek Pilát; Alexandr Jonás; Pavel Zemánek; Mojmir Sery; Jan Jezek; Silvie Bernatová; Ladislav Nedbal; Martin Trtílek
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Paper Abstract

The ability to identify and characterize microorganisms (algae, bacteria, eukaryotic cells) from minute sample volumes in a rapid and reliable way is the crucial first step in their classification and characterization. In the light of this challenge related to microorganisms exploitation Raman spectroscopy can be used as a powerful tool for chemical analysis. Raman spectroscopy can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Moreover, Raman spectroscopy can be combined with optical tweezers and with microfluidic chips to measure nutrient dynamics and metabolism in vivo, in real-time, and label free. We demonstrate the feasibility to employ Raman spectroscopy-based sensor to sort microorganisms (bacteria, algae) according to the Raman spectra. It is now quite feasible to sort algal cells according to the degree of unsaturation (iodine value) in lipid storage bodies.

Paper Details

Date Published: 9 September 2011
PDF: 7 pages
Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970F (9 September 2011); doi: 10.1117/12.893420
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdenek Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Alexandr Jonás, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmir Sery, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ladislav Nedbal, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Martin Trtílek, Photon Systems Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 8097:
Optical Trapping and Optical Micromanipulation VIII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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