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Proceedings Paper

High-speed tracking of intracellular structures: understanding the transport mechanisms in living plant cells
Author(s): C. López-Quesada; M. Joseph; J. Selva; A. Farré; G. Egea; M. D. Ludevid; E. Martín-Badosa; M. Montes-Usategui
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Paper Abstract

Zera® technology offers the possibility to artificially induce the formation of spherical organelles in different kinds of cells. Their large size and high density, compared to the native organelles of the cell, make them good candidates to be used as a handles for the realization of biophysical experiments inside living cells. Furthermore, they present a high signal-to-noise ratio in fluorescence microscopy and small photobleaching. This work focuses mainly on the nature of protein body motion in Nicotiana Benthamiana (tobacco) cells. The high-speed tracking of these structures reveals that they move in a stepwise mode, suggesting that myosin XI motors directly pull these organelles through the cytosol. Our results indicate that these artificially-induced structures are well integrated into the natural processes of the cell so that the technique may be advantageous for the study of the intracellular transport mechanisms. Large forces can be exerted with optical tweezers to mechanically interact with the moving organelles.

Paper Details

Date Published: 9 September 2011
PDF: 11 pages
Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970G (9 September 2011); doi: 10.1117/12.893384
Show Author Affiliations
C. López-Quesada, Univ. de Barcelona (Spain)
M. Joseph, Consejo Superior de Investigaciones Científicas (Spain)
J. Selva, Univ. de Barcelona (Spain)
A. Farré, Univ. de Barcelona (Spain)
G. Egea, Univ. de Barcelona (Spain)
M. D. Ludevid, Consejo Superior de Investigaciones Científicas (Spain)
E. Martín-Badosa, Univ. de Barcelona (Spain)
M. Montes-Usategui, Univ. de Barcelona (Spain)


Published in SPIE Proceedings Vol. 8097:
Optical Trapping and Optical Micromanipulation VIII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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