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Proceedings Paper

Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)
Author(s): Michelle M. Packard; Maxim Shusteff; Evangelyn Alocilja
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Paper Abstract

Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times. The assay described in this work incorporates on-chip dielectrophoretic capture and concentration of bacterial cells, thermal lysis, cell permeabilization, and nucleic acid denaturation and fluorescence resonance energy transfer assisted in-situ hybridization (FRET-ISH) species identification. Identification is achieved completely on chip in less than thirty minutes from receipt of sample compared to multiple hours required by traditional RT-PCR and its requisite sample preparation.

Paper Details

Date Published: 12 September 2011
PDF: 6 pages
Proc. SPIE 8099, Biosensing and Nanomedicine IV, 80990J (12 September 2011); doi: 10.1117/12.892425
Show Author Affiliations
Michelle M. Packard, Michigan State Univ. (United States)
Lawrence Livermore National Lab. (United States)
Maxim Shusteff, Lawrence Livermore National Lab. (United States)
Evangelyn Alocilja, Michigan State Univ. (United States)

Published in SPIE Proceedings Vol. 8099:
Biosensing and Nanomedicine IV
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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