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Proceedings Paper

Design considerations and performance of a compact LED illuminator for microscopes
Author(s): Jorge Monteiro; Sushil Kumar Mendiratta; João C. Cascalheira
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Paper Abstract

Conventional LED based illuminating systems for microscopes are either ring illuminators or expensive fiber optics based sources to input the light at the conventional places. Here we describe a different design strategy that leads to a compact, economical, relatively cool and more versatile source that is usable in the transmission as well as the reflection configuration; the module can be easily configured for discrete wavelengths for fluorescence markers and thus is suitable for biology related applications. We show the performance characteristics of the module that we have developed. Comparison measurements show that led based source that we have developed has adequate homogeneity of the illumination field, white leds lead to better contrast and use of narrow band leds have significant advantages over the conventional approach in fluorescence microscopy applications.

Paper Details

Date Published: 26 July 2011
PDF: 6 pages
Proc. SPIE 8001, International Conference on Applications of Optics and Photonics, 80014B (26 July 2011); doi: 10.1117/12.892204
Show Author Affiliations
Jorge Monteiro, Univ. of Aveiro (Portugal)
Sushil Kumar Mendiratta, Univ. of Aveiro (Portugal)
João C. Cascalheira, Bioptica (Portugal)

Published in SPIE Proceedings Vol. 8001:
International Conference on Applications of Optics and Photonics
Manuel Filipe Costa, Editor(s)

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