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Proceedings Paper

Detection and visualization improvement of spermatozoa cells by digital holography
Author(s): L. Miccio; A. Finizio; P. Memmolo; M. Paturzo; F. Merola; G. Coppola; G. Di Caprio; M. Gioffrè; R. Puglisi; D. Balduzzi; A. Galli; P. Ferraro
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Paper Abstract

Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels.

Paper Details

Date Published: 14 June 2011
PDF: 6 pages
Proc. SPIE 8089, Molecular Imaging III, 80890C (14 June 2011); doi: 10.1117/12.889972
Show Author Affiliations
L. Miccio, Istituto Nazionale di Ottica Applicata, CNR (Italy)
A. Finizio, Istituto Nazionale di Ottica Applicata, CNR (Italy)
P. Memmolo, Istituto Nazionale di Ottica Applicata, CNR (Italy)
M. Paturzo, Istituto Nazionale di Ottica Applicata, CNR (Italy)
F. Merola, Istituto Nazionale di Ottica Applicata, CNR (Italy)
G. Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
G. Di Caprio, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
M. Gioffrè, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
R. Puglisi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
D. Balduzzi, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
A. Galli, Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy)
P. Ferraro, Istituto Nazionale di Ottica Applicata, CNR (Italy)


Published in SPIE Proceedings Vol. 8089:
Molecular Imaging III
Charles P. Lin; Vasilis Ntziachristos, Editor(s)

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