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Proceedings Paper

Application of oblique plane microscopy to high speed live cell imaging
Author(s): Sunil Kumar; Dean Wilding; Markus B. Sikkel; Alexander R. Lyon; Ken T. MacLeod; Chris Dunsby
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Paper Abstract

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. We present high speed 2D and 3D optically sectioned OPM imaging of live cells using a high NA water immersion lens.

Paper Details

Date Published: 15 June 2011
PDF: 5 pages
Proc. SPIE 8086, Advanced Microscopy Techniques II, 80860V (15 June 2011); doi: 10.1117/12.889841
Show Author Affiliations
Sunil Kumar, Imperial College London (United Kingdom)
Dean Wilding, Imperial College London (United Kingdom)
Markus B. Sikkel, Imperial College London (United Kingdom)
Alexander R. Lyon, Imperial College London (United Kingdom)
Ken T. MacLeod, Imperial College London (United Kingdom)
Chris Dunsby, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 8086:
Advanced Microscopy Techniques II
Peter T. C. So; Emmanuel Beaurepaire, Editor(s)

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