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Proceedings Paper

In vivo laser assisted end-to-end anastomosis with ICG-infused chitosan patches
Author(s): Francesca Rossi; Paolo Matteini; Giuseppe Esposito; Alba Scerrati; Alessio Albanese; Alfredo Puca; Giulio Maira; Giacomo Rossi; Roberto Pini
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Paper Abstract

Laser assisted vascular repair is a new optimized technique based on the use of ICG-infused chitosan patch to close a vessel wound, with or even without few supporting single stitches. We present an in vivo experimental study on an innovative end-to-end laser assisted vascular anastomotic (LAVA) technique, performed with the application of ICGinfused chitosan patches. The photostability and the mechanical properties of ICG-infused chitosan films were preliminary measured. The in vivo study was performed in 10 New Zealand rabbits. After anesthesia, a 3-cm segment of the right common carotid artery was exposed, thus clamped proximally and distally. The artery was then interrupted by means of a full thickness cut. Three single microsutures were used to approximate the two vessel edges. The ICG-infused chitosan patch was rolled all over the anastomotic site and welded by the use of a diode laser emitting at 810 nm and equipped with a 300 μm diameter optical fiber. Welding was obtained by delivering single laser spots to induce local patch/tissue adhesion. The result was an immediate closure of the anastomosis, with no bleeding at clamps release. Thus animals underwent different follow-up periods, in order to evaluate the welded vessels over time. At follow-up examinations, all the anastomoses were patent and no bleeding signs were documented. Samples of welded vessels underwent histological examinations. Results showed that this technique offer several advantages over conventional suturing methods: simplification of the surgical procedure, shortening of the operative time, better re-endothelization and optimal vascular healing process.

Paper Details

Date Published: 2 June 2011
PDF: 4 pages
Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 809212 (2 June 2011); doi: 10.1117/12.889800
Show Author Affiliations
Francesca Rossi, Institute of Applied Physics Nello Carrara, CNR (Italy)
Paolo Matteini, Institute of Applied Physics Nello Carrara, CNR (Italy)
Giuseppe Esposito, Catholic Univ. (Italy)
Alba Scerrati, Catholic Univ. (Italy)
Alessio Albanese, Catholic Univ. (Italy)
Alfredo Puca, Catholic Univ. (Italy)
Giulio Maira, Catholic Univ. (Italy)
Giacomo Rossi, Univ. of Camerino (Italy)
Roberto Pini, Institute of Applied Physics Nello Carrara, CNR (Italy)


Published in SPIE Proceedings Vol. 8092:
Medical Laser Applications and Laser-Tissue Interactions V
Ronald Sroka; Lothar D. Lilge, Editor(s)

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