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Proceedings Paper

Morphological and molecular analysis of the collagen fibers in inflammatory process
Author(s): Luis Felipe das Chagas e Silva de Carvalho; Mônica Ghislaine Oliveira Alves; Carlos Alexandre Soares; Janete Dias Almeida; Herculano da Silva Martinho
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Paper Abstract

Collagen makes up one third of the total protein in humans, being formed by the connection of three polypeptide chains arranged in a triple helix. This protein has fundamental importance in the formation of extracellular matrix of connective tissue. This study aimed to analyze the structural changes of collagen, which are resulting from inflammatory processes in oral mucosa, and to make the comparative analysis between the histopathology and the Raman spectra. The samples of tissues with inflammatory fibrous hyperplasia (IFH) and normal mucosa (NM) were evaluated by Raman Spectroscopy, hematoxylin-eosin and Massons trichrome stain. The histological analysis in both stains showed differences in collagen fibers, which was presented as thin fibers and arranged in parallel direction in NM and as collagen fibers are thick, mature and not organized, showing that these types of stain show morphological changes of collagen in IFH. The Raman Spectroscopy discriminate the groups of NM and IFH based on vibrational modes of proline, hydroxiproline and CH3, CH2. The histological stains only shows information from morphological data, and can be complemented by Raman spectra. This technique could demonstrate that inflammatory process caused some changes in collagen structure which is related to aminoacids such as proline and hidroxyproline.

Paper Details

Date Published: 8 June 2011
PDF: 7 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808712 (8 June 2011); doi: 10.1117/12.889747
Show Author Affiliations
Luis Felipe das Chagas e Silva de Carvalho, Univ. Federal do ABC (Brazil)
Univ. Estadual Paulista (Portugal)
Mônica Ghislaine Oliveira Alves, Univ. Estadual Paulista (Portugal)
Carlos Alexandre Soares, Univ. Federal do ABC (Brazil)
Janete Dias Almeida, Univ. Estadual Paulista (Portugal)
Herculano da Silva Martinho, Univ. Federal do ABC (Brazil)


Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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