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Proceedings Paper

Nonlinear optical molecular imaging enables metabolic redox sensing in tissue-engineered constructs
Author(s): Leng-Chun Chen; William R. Lloyd; Robert H. Wilson; Shiuhyang Kuo; Cynthia L. Marcelo; Stephen E. Feinberg; Mary-Ann Mycek
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Paper Abstract

Tissue-engineered constructs require noninvasive monitoring of cellular viability prior to implantation. In a preclinical study on human Ex Vivo Produced Oral Mucosa Equivalent (EVPOME) constructs, nonlinear optical molecular imaging was employed to extract morphological and functional information from intact constructs. Multiphoton excitation fluorescence images were acquired using endogenous fluorescence from cellular nicotinamide adenine dinucleotide phosphate [NAD(P)H] and flavin adenine dinucleotide (FAD). The images were analyzed to report quantitatively on tissue structure and metabolism (redox ratio). Both thickness variations over time and cell distribution variations with depth were identified, while changes in redox were quantified. Our results show that nonlinear optical molecular imaging has the potential to visualize and quantitatively monitor the growth and viability of a tissue-engineered construct over time.

Paper Details

Date Published: 14 June 2011
PDF: 7 pages
Proc. SPIE 8089, Molecular Imaging III, 80890J (14 June 2011); doi: 10.1117/12.889713
Show Author Affiliations
Leng-Chun Chen, Univ. of Michigan (United States)
William R. Lloyd, Univ. of Michigan (United States)
Robert H. Wilson, Univ. of Michigan (United States)
Shiuhyang Kuo, Univ. of Michigan (United States)
Cynthia L. Marcelo, Univ. of Michigan (United States)
Stephen E. Feinberg, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 8089:
Molecular Imaging III
Charles P. Lin; Vasilis Ntziachristos, Editor(s)

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