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Proceedings Paper

Multimodal nonlinear optical microscopy used to discriminate epithelial ovarian cancer
Author(s): J. Adur; V. B. Pelegati; A. A. de Thomaz; D. B. Almeida; F. Bottcher-Luiz; L. A. L. A. Andrade; C. L. Cesar
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Paper Abstract

We used human specimens of epithelial ovarian cancer (serous type) to test the feasibility of nonlinear imaging as complementary tools for ovarian cancer diagnosis. Classical hematoxylin-and-eosin stained sections were applied to combining two-photon excitation fluorescence (TPEF), second (SHG), and third (THG) harmonic microscopy within the same imaging platform. We show that strong TPEF + SHG + THG signals can be obtained in fixed samples stained with Hematoxylin & Eosin (H&E) stored for a very long time and that H&E staining enhanced the THG signal. We demonstrate using anisotropy and morphological measurements, that SHG and THG of stained optical sections allow reproducible identification of neoplastic features such as architectural alterations of collagen fibrils at different stages of the neoplastic transformation and cellular atypia. Taken together, these results suggest that, with our viable imaging system, we can qualitatively and quantitatively assess endogenous optical biomarkers of the ovarian tissue with SHG and THG microscopy. This imaging capability may prove to be highly valuable in aiding to determine structural changes at the cellular and tissue levels, which may contribute to the development of new diagnostic techniques.

Paper Details

Date Published: 8 June 2011
PDF: 7 pages
Proc. SPIE 8086, Advanced Microscopy Techniques II, 80860L (8 June 2011); doi: 10.1117/12.889554
Show Author Affiliations
J. Adur, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
Lab. de Microscopia Aplicada a Estudios Moleculares y Celulares, Univ. Nacional de Entre Rios (Argentina)
V. B. Pelegati, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
A. A. de Thomaz, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
D. B. Almeida, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)
F. Bottcher-Luiz, Univ. Estadual de Campinas (Brazil)
L. A. L. A. Andrade, Univ. Estadual de Campinas (Brazil)
C. L. Cesar, Lab. de Aplicações Biomédicas de Lasers, Instituto de Física, UNICAMP (Brazil)


Published in SPIE Proceedings Vol. 8086:
Advanced Microscopy Techniques II
Peter T. C. So; Emmanuel Beaurepaire, Editor(s)

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