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Proceedings Paper

Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms
Author(s): S. Daddi Oubekka; R. Briandet; F. Waharte; M.-P. Fontaine-Aupart; K. Steenkeste
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Paper Abstract

The diffusion capabilities of free fluorophores inside the heterogeneous three dimensional structure of Staphylococcus aureus biofilm were studied by an original image-based Fluorescence Recovery After Photobleaching method. The study was extended to BODIPY-vancomycin in order to better understand the mechanisms involved in the high tolerance of the bacteria embedded in a biofilm to the antibiotic.

Paper Details

Date Published: 10 June 2011
PDF: 7 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871I (10 June 2011); doi: 10.1117/12.889461
Show Author Affiliations
S. Daddi Oubekka, Institut des Sciences Moléculaires d'Orsay, CNRS, Univ. Paris-Sud (France)
R. Briandet, INRA (France)
AgroParisTech (France)
F. Waharte, CNRS - Institut Curie (France)
M.-P. Fontaine-Aupart, Institut des Sciences Moléculaires d'Orsay, CNRS, Univ. Paris-Sud (France)
K. Steenkeste, Institut des Sciences Moléculaires d'Orsay, CNRS, Univ. Paris-Sud (France)


Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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