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Proceedings Paper

Lensfree in-line holographic detection of bacteria
Author(s): V. Poher; C. P. Allier; J. G. Coutard; L. Hervé; J. M. Dinten
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Paper Abstract

Due to low light scattering, bacteria are difficult to detect using lensless imaging systems. In order to detect individual bacteria, we report a method based on a thin wetting film imaging that produces a micro-lens effect on top of each bacterium when the sample dries up. The imaging using a high-end CMOS sensor is combined with an in-line holographic reconstruction to improve positive detection rate up to 95% with micron-sized beads at high density of ~103 objects/mm2. The system allows detecting from single bacterium to densely packed objects (103 bacteria/μl) within 10μl sample. As an example, E.coli, Bacillus subtilis and Bacillus thuringiensis, has been successfully detected with strong signal to noise ratio across a 24mm2 field of view.

Paper Details

Date Published: 9 June 2011
PDF: 7 pages
Proc. SPIE 8086, Advanced Microscopy Techniques II, 808619 (9 June 2011); doi: 10.1117/12.889324
Show Author Affiliations
V. Poher, CEA, LETI, MINATEC (France)
C. P. Allier, CEA, LETI, MINATEC (France)
J. G. Coutard, CEA, LETI, MINATEC (France)
L. Hervé, CEA, LETI, MINATEC (France)
J. M. Dinten, CEA, LETI, MINATEC (France)

Published in SPIE Proceedings Vol. 8086:
Advanced Microscopy Techniques II
Peter T. C. So; Emmanuel Beaurepaire, Editor(s)

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