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Proceedings Paper

Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography
Author(s): Rakesh Patalay; Clifford Talbot; Yuriy Alexandrov; Ian Munro; Hans Georg Breunig; Karsten König; Sean Warren; Mark A. A. Neil; Paul M. W. French; Anthony Chu; Gordon W. Stamp; Christopher Dunsby
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Paper Abstract

Multispectral fluorescence lifetime imaging (FLIM) using two photon microscopy as a non-invasive technique for the diagnosis of skin lesions is described. Skin contains fluorophores including elastin, keratin, collagen, FAD and NADH. This endogenous contrast allows tissue to be imaged without the addition of exogenous agents and allows the in vivo state of cells and tissues to be studied. A modified DermaInspect® multiphoton tomography system was used to excite autofluorescence at 760 nm in vivo and on freshly excised ex vivo tissue. This instrument simultaneously acquires fluorescence lifetime images in four spectral channels between 360-655 nm using time-correlated single photon counting and can also provide hyperspectral images. The multispectral fluorescence lifetime images were spatially segmented and binned to determine lifetimes for each cell by fitting to a double exponential lifetime model. A comparative analysis between the cellular lifetimes from different diagnoses demonstrates significant diagnostic potential.

Paper Details

Date Published: 14 June 2011
PDF: 8 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808718 (14 June 2011); doi: 10.1117/12.889314
Show Author Affiliations
Rakesh Patalay, Imperial College London (United Kingdom)
Imperial College Healthcare NHS Trust (United Kingdom)
Clifford Talbot, Imperial College London (United Kingdom)
Yuriy Alexandrov, Imperial College London (United Kingdom)
Ian Munro, Imperial College London (United Kingdom)
Hans Georg Breunig, JenLab GmbH (Germany)
Karsten König, JenLab GmbH (Germany)
Sean Warren, Imperial College London (United Kingdom)
Mark A. A. Neil, Imperial College London (United Kingdom)
Paul M. W. French, Imperial College London (United Kingdom)
Anthony Chu, Imperial College Healthcare NHS Trust (United Kingdom)
Gordon W. Stamp, Imperial College Healthcare NHS Trust (United Kingdom)
CRUK London Research Institute (United Kingdom)
Christopher Dunsby, Imperial College London (United Kingdom)
Imperial College Healthcare NHS Trust (United Kingdom)


Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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