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Proceedings Paper

Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope
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Paper Abstract

We demonstrate a modular and versatile experimental setup that enables straightforward compression (and then shaping) of ultrashort laser pulses at the imaging plane of a two-photon microscope. A commercially available pulse shaper is used in conjunction with a commercially available broadband Ti:Sapphire oscillator to produce sub-8fs pulses at the focus of a high-numerical-aperture objective. Automated adaptive pulse compression, based on multiphoton intrapulse interference phase scan (MIIPS), is verified in situ by shaper assisted interferometric autocorrelation. Two-photon excited fluorescence image of a mouse kidney slide is obtained to confirm microscopic sectioning capabilities.

Paper Details

Date Published: 1 March 2011
PDF: 4 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79033B (1 March 2011); doi: 10.1117/12.889223
Show Author Affiliations
Dmitry Pestov, Biophotonic Solutions, Inc. (United States)
Bingwei Xu, Biophotonic Solutions, Inc. (United States)
Haowen Li, Biophotonic Solutions, Inc. (United States)
Marcos Dantus, Biophotonic Solutions, Inc. (United States)
Michigan State Univ. (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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