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Proceedings Paper

Study on suitable for regeneration system of genetic transformation of kiwifruit
Author(s): Yun Xiang Yuan
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Paper Abstract

The stems of Actinidia Qinmei were taken as explants and induced callus formation after having gained the aseptic seedling in primary culture successfully, and then the calli were placed on different combinations regeneration medium. The results showed that the induction medium added 1 mg/L 6-BA and 0.1 mg/L NAA was beneficial to the callus induction in dark culture condition. The suitable regeneration medium was MS containing 2.0 mg/L 6BA, 0.1 mg/L NAA and 3% (W/V) sucrose, it could improve obviously the frequency of regenerated shoots. This method separated the callus induction from shoot regeneration and obtained more callus to optimize the regenerated medium, and also was advantageous to Kiwifruit genetic transformation.

Paper Details

Date Published: 4 February 2011
PDF: 5 pages
Proc. SPIE 7752, PIAGENG 2010: Photonics and Imaging for Agricultural Engineering, 775206 (4 February 2011); doi: 10.1117/12.887367
Show Author Affiliations
Yun Xiang Yuan, Weinan Teachers Univ. (China)


Published in SPIE Proceedings Vol. 7752:
PIAGENG 2010: Photonics and Imaging for Agricultural Engineering
Honghua Tan, Editor(s)

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