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Proceedings Paper

Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells
Author(s): Anupam K. Misra; Lori E. Kamemoto; Ningjie Hu; Ava C. Dykes; Qigui Yu; Pavel V. Zinin; Shiv K. Sharma
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Paper Abstract

Micro- Raman spectroscopic investigation of ALVAC virus and of normal chicken embryo fibroblast cells and the cells infected with ALVAC virus labeled with green fluorescence protein (GFP) were performed with a 785 nm laser. Good quality Micro-Raman spectra of the Alvac II virus were obtained. These spectra show that the ALVAC II virus contains buried tyrosine residues and the coat protein of the virus has α-helical structure. A comparison of Raman spectra of normal and virus infected chicken embryo fibroblast cells revealed that the virus infected cells show additional bands at 535, 928, and 1091 cm-1, respectively, corresponding to δ(C-O-C) glycosidic ring, protein α-helix, and DNA (O-P-O) modes. In addition, the tyrosine resonance double (833 and 855 cm-1) shows reversal in the intensity of the higher-frequency band as compared to the normal cells that can be used to identify the infected cells. In the C-H stretching region, the infected cells show bands with higher intensity as compared to that of the corresponding bands in the normal cells. We also found that the presence of GFP does not affect the Raman spectra of samples when using a 785 nm micro-Raman system because the green fluorescence wavelength of GFP is well below the Stokes-Raman shifted spectral region.

Paper Details

Date Published: 26 May 2011
PDF: 13 pages
Proc. SPIE 8025, Smart Biomedical and Physiological Sensor Technology VIII, 80250C (26 May 2011); doi: 10.1117/12.887165
Show Author Affiliations
Anupam K. Misra, Univ. of Hawai'i (United States)
Lori E. Kamemoto, John A. Burns School of Medicine, Univ. of Hawai'i (United States)
Ningjie Hu, Indiana Univ. School of Medicine (United States)
Ava C. Dykes, Univ. of Hawai'i (United States)
Qigui Yu, Indiana Univ. School of Medicine (United States)
Pavel V. Zinin, Univ. of Hawai'i (United States)
Shiv K. Sharma, Univ. of Hawai'i (United States)

Published in SPIE Proceedings Vol. 8025:
Smart Biomedical and Physiological Sensor Technology VIII
Brian M. Cullum; Eric S. McLamore, Editor(s)

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