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Proceedings Paper

Amplification-free point of care immunosensor for detecting type V collagen at a concentration level of ng/ml
Author(s): Pei-Yu Chung; Evelyn R. Bracho-Sanchez; Peng Jiang; JeanClare Seagrave; Matthew R. Duncan; Gary R. Grotendorst; Gregory Schultz; Christopher Batich
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Paper Abstract

Point-of-care testing (POCT) is applicable in the immediate vicinity of the patient, where timely diagnosis or prognostic information could help doctors decide the following treatment. Among types of developed POCT, gold nanoparticle based lateral flow strip technology provides advantages such as simple operation, cost-effectiveness, and a user-friendly platform. Therefore, this type of POCT is most likely to be used in battlefields and developing countries. However, conventional lateral flow strips suffer from low detection limits. Although enzyme-linked amplification was demonstrated to improve the detection limit and sensitivity by stronger visible lines or by permitting electrochemical analytical instrumentation, the enzyme labels have potential to cause interference with other enzymes in our body fluids. To eliminate this limitation, we developed an amplification-free gold nanoparticle-based immunosensor applied for detecting collagen type V, which is produced or released abnormally during rejection of lung transplants and sulfur mustard exposure. By using suitable blocking protein to stabilize gold nanoparticles as the reporter probe, a low detection limit of ng/ml was achieved. This strategy is a promising platform for clinical POCT, with potential applications in military or disaster response.

Paper Details

Date Published: 16 May 2011
PDF: 7 pages
Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 80291C (16 May 2011); doi: 10.1117/12.887147
Show Author Affiliations
Pei-Yu Chung, Univ. of Florida (United States)
Evelyn R. Bracho-Sanchez, Univ. of Florida (United States)
Peng Jiang, Univ. of Florida (United States)
JeanClare Seagrave, Lovelace Respiratory Research Institute (United States)
Matthew R. Duncan, Lovelace Respiratory Research Institute (United States)
Gary R. Grotendorst, Lovelace Respiratory Research Institute (United States)
Gregory Schultz, Univ. of Florida (United States)
Christopher Batich, Univ. of Florida (United States)


Published in SPIE Proceedings Vol. 8029:
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII
B. V. K. Vijaya Kumar; Sárka O. Southern; Kevin N. Montgomery; Salil Prabhakar; Arun A. Ross; Carl W. Taylor; Bernhard H. Weigl, Editor(s)

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