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Proceedings Paper

Raman microspectroscopy based sensor of algal lipid unsaturation
Author(s): Ota Samek; Zdeněk Pilát; Alexandr Jonáš; Pavel Zemánek; Mojmír Šerý; Jan Ježek; Silvie Bernatová; Ladislav Nedbal; Martin Trtílek
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Paper Abstract

Raman spectroscopy is a powerful tool for chemical analysis. This technique can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Therefore, Raman spectroscopy can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy can be combined with optical tweezers. We have employed microfluidic system to deliver the sampled microalgae to the Raman-tweezers. This instrument is able to measure chemical composition of cells and to track metabolic processes in vivo, in real-time and label-free making it possible to detect population variability in a wide array of traits. Moreover, employing an active sorting switch, cells can be separated depending on input parameters obtained from Raman spectra. We focus on algal lipids which are promising potential products for biofuel as well as for nutrition. Important parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids. We demonstrate the capacity of our Raman tweezers based sensor to sort cells according to the degree of unsaturation in lipid storage bodies of individual living algal cells.

Paper Details

Date Published: 7 May 2011
PDF: 6 pages
Proc. SPIE 8073, Optical Sensors 2011; and Photonic Crystal Fibers V, 80730O (7 May 2011); doi: 10.1117/12.886750
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Alexandr Jonáš, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Mojmír Šerý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ladislav Nedbal, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Martin Trtílek, Photon Systems Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 8073:
Optical Sensors 2011; and Photonic Crystal Fibers V
Kyriacos Kalli; Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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