Share Email Print
cover

Proceedings Paper

DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre
Author(s): Monika Rutowska; Jiadi Lu; Fatima C. Garcia-Gunning; Andrew D. Ellis
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

Paper Details

Date Published: 17 May 2011
PDF: 4 pages
Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536I (17 May 2011); doi: 10.1117/12.886070
Show Author Affiliations
Monika Rutowska, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)
Jiadi Lu, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)
Fatima C. Garcia-Gunning, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)
Andrew D. Ellis, Tyndall National Institute (Ireland)
Univ. College Cork (Ireland)


Published in SPIE Proceedings Vol. 7753:
21st International Conference on Optical Fiber Sensors
Jacques Albert; Wojtek J. Bock, Editor(s)

© SPIE. Terms of Use
Back to Top