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Proceedings Paper

Diagnostic protein biomarkers for severe, moderate and mild traumatic brain injury
Author(s): Jackson Streeter; Ronald L Hayes; Kevin K. W. Wang
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Paper Abstract

Traumatic Brain Injury (TBI) is a major problem in military and civilian medicine. Yet, there are no simple non-invasive diagnostics for TBI. Our goal is to develop and clinically validate blood-based biomarker assays for the diagnosis, prognosis and management of mild, moderate and severe TBI patients. These assays will ultimately be suitable for deployment to far-forward combat environments. Using a proteomic and systems biology approach, we identified over 20 candidate biomarkers for TBI and developed robust ELISAs for at least 6 candidate biomarkers, including Ubiquitin C-terminal hydrolase- L1 (UCH-L1), Glial Fibrillary Acidic Protein (GFAP) and a 145 kDa breakdown products of αII-spectrin (SBDP 145) generated by calpain proteolysis. In a multi-center feasibility study (Biomarker Assessment For Neurotrauma Diagnosis And Improved Triage System (BANDITS), we analyzed CSF and blood samples from 101 adult patients with severe TBI [Glasgow Coma Scale (GCS) ≤ 8] at 6 sites and analyzed 27 mild TBI patients and 5 moderate TBI patients [GCS 9-15] from 2 sites in a pilot study. We identified that serum levels of UCH-L1, GFAP and SBDP145 have strong diagnostic and prognostic properties for severe TBI over controls. Similarly initial post-TBI serum levels (< 6 h) of UCH-L1 and GFAP have diagnostic characteristics for moderate and mild TBI. We are now furthering assay production, refining assay platforms (both benchtop and point-ofcare/ handheld) and planning a pivotal clinical study to seek FDA approval of these TBI diagnostic assays.

Paper Details

Date Published: 16 May 2011
PDF: 16 pages
Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 80290N (16 May 2011); doi: 10.1117/12.885615
Show Author Affiliations
Jackson Streeter, Banyan Biomarkers, Inc. (United States)
Ronald L Hayes, Banyan Biomarkers, Inc. (United States)
Kevin K. W. Wang, Banyan Biomarkers, Inc. (United States)


Published in SPIE Proceedings Vol. 8029:
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII
B. V. K. Vijaya Kumar; Sárka O. Southern; Kevin N. Montgomery; Salil Prabhakar; Arun A. Ross; Carl W. Taylor; Bernhard H. Weigl, Editor(s)

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