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Proceedings Paper

Confocal axial beam scanning through LC-SLM
Author(s): Limin Zou; Jin Dong; Zhigang Fan
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Paper Abstract

The axial beam scanning in confocal microscopy has been implemented using liquid crystal spatial light modulator (LC-SLM). A zoom illuminating lens is realized by introducing LC-SLM into the confocal illumination light path, and thus it shifts the focus of the objective lens axially. The axial optical sectioning in a conventional confocal microscope is hereby achieved by beam scanning rather than mechanically moving the objective lens. The axial focus shift of the objective lens is realized by changing the modulation phase bitmaps of LC-SLM. By electrically controlling LC-SLM, it has been demonstrated that the focus can shift in the axial direction continuously over a 100μm range, while the FWHM of the focal spot keeps the same as that without LC-SLM. Experimental results further show that the axial intensity curves shift axially in confocal microscopy and confocal axial beam scanning replacing mechanical scanning can therefore be implemented.

Paper Details

Date Published: 28 December 2010
PDF: 6 pages
Proc. SPIE 7544, Sixth International Symposium on Precision Engineering Measurements and Instrumentation, 75442F (28 December 2010); doi: 10.1117/12.885455
Show Author Affiliations
Limin Zou, Harbin Institute of Technology (China)
Jin Dong, Harbin Institute of Technology (China)
Zhigang Fan, Harbin Institute of Technology (China)

Published in SPIE Proceedings Vol. 7544:
Sixth International Symposium on Precision Engineering Measurements and Instrumentation
Jiubin Tan; Xianfang Wen, Editor(s)

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