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Proceedings Paper

Digital holographic microscopy of optically trapped three-dimensional microstructures
Author(s): Ali-Reza Moradi; Arun Anand; Mohammad Kutub Ali; Bahram Javidi
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Paper Abstract

Digital holographic microscopy (DHM) provides a non-destructive and quantitative phase contrast imaging that is suitable for high resolving investigations of living cells. On the other hand, for many applications including cell analysis the immobilization of the sample under study is a crucial task. Optical trap is an elegant candidate to immobilize the micro samples. In this work, DHM is integrated with an optical trapping setup. This combination is of particular advantage for quantitative visualization of three dimensional structures that are trapped by laser beam. The recorded hologram by CCD can be post-processed to three dimensional (3D) reconstruction of the trapped objects.

Paper Details

Date Published: 20 May 2011
PDF: 5 pages
Proc. SPIE 8043, Three-Dimensional Imaging, Visualization, and Display 2011, 804310 (20 May 2011); doi: 10.1117/12.885192
Show Author Affiliations
Ali-Reza Moradi, Zanjan Univ. (Iran, Islamic Republic of)
Institute for Advanced Studies in Basic Sciences (Iran, Islamic Republic of)
Arun Anand, The Maharaja Sayajirao Univ. of Baroda (India)
Mohammad Kutub Ali, Institute for Advanced Studies in Basic Sciences (Iran, Islamic Republic of)
Bahram Javidi, Univ. of Connecticut (United States)

Published in SPIE Proceedings Vol. 8043:
Three-Dimensional Imaging, Visualization, and Display 2011
Bahram Javidi; Jung-Young Son, Editor(s)

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