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Proceedings Paper

Nanomaterial strategies for immunodetection
Author(s): M. D. Porter; M. C. Granger; L. M. Siperko; R. J. Lipert
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Paper Abstract

Metallic nanoparticles are playing increasingly important roles in biodiagnostic platforms. This emergence reflects the need to detect disease indicating entities at increasingly lower levels in human and veterinary diagnostics, homeland security, and food and water safety. To establish this perspective, this paper overviews our recent work using surface enhanced Raman scattering for detection of proteins, viruses, and microorganisms in heterogeneous immunoassays. It describes the assay platform, which is comprised of an antibody-modified capture substrate and gold nanoparticle-based label. The latter draws on the ability to reproducibly construct gold nanoparticles modified with a monolayer of an intrinsically strong Raman scatterer that is then coated with a layer of antibodies. This construct, referred to as an extrinsic Raman label, takes advantage of the signal enhancement of scatterers when coated on nanometer-sized gold particles and the antigenic binding specificity of the immobilized antibody layer. Challenges related to nonspecific adsorption, particle stability, and measurement reproducibility are also briefly examined.

Paper Details

Date Published: 13 May 2011
PDF: 12 pages
Proc. SPIE 8031, Micro- and Nanotechnology Sensors, Systems, and Applications III, 80311T (13 May 2011); doi: 10.1117/12.884909
Show Author Affiliations
M. D. Porter, The Univ. of Utah (United States)
M. C. Granger, The Univ. of Utah (United States)
L. M. Siperko, The Univ. of Utah (United States)
R. J. Lipert, Iowa State Univ. (United States)


Published in SPIE Proceedings Vol. 8031:
Micro- and Nanotechnology Sensors, Systems, and Applications III
Thomas George; M. Saif Islam; Achyut K. Dutta, Editor(s)

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