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Proceedings Paper

Development of narrow-band fluorescence index for the detection of aflatoxin contaminated corn
Author(s): Haibo Yao; Zuzana Hruska; Russell Kincaid; Ambrose Ononye; Robert L. Brown; Deepak Bhatnagar; Thomas E. Cleveland
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Paper Abstract

Aflatoxin is produced by the fungus Aspergillus flavus when the fungus invades developing corn kernels. Because of its potent toxicity, the levels of aflatoxin are regulated by the Food and Drug Administration (FDA) in the US, allowing 20 ppb (parts per billion) limits in food, and feed intended for interstate commerce. Currently, aflatoxin detection and quantification methods are based on analytical tests. These tests require the destruction of samples, can be costly and time consuming, and often rely on less than desirable sampling techniques. Thus, the ability to detect aflatoxin in a rapid, non-invasive way is crucial to the corn industry in particular. This paper described how narrow-band fluorescence indices were developed for aflatoxin contamination detection based on single corn kernel samples. The indices were based on two bands extracted from full wavelength fluorescence hyperspectral imagery. The two band results were later applied to two large sample experiments with 25 g and 1 kg of corn per sample. The detection accuracies were 85% and 95% when 100 ppb threshold was used. Since the data acquisition period is significantly lower for several image bands than for full wavelength hyperspectral data, this study would be helpful in the development of real-time detection instrumentation for the corn industry.

Paper Details

Date Published: 2 June 2011
PDF: 9 pages
Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 80270D (2 June 2011); doi: 10.1117/12.884400
Show Author Affiliations
Haibo Yao, Mississippi State Univ. (United States)
Zuzana Hruska, Mississippi State Univ. (United States)
Russell Kincaid, Mississippi State Univ. (United States)
Ambrose Ononye, Mississippi State Univ. (United States)
Robert L. Brown, U.S.D.A. Agricultural Research Service (United States)
Deepak Bhatnagar, U.S.D.A. Agricultural Research Service (United States)
Thomas E. Cleveland, U.S.D.A. Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 8027:
Sensing for Agriculture and Food Quality and Safety III
Moon S. Kim; Shu-I Tu; Kaunglin Chao, Editor(s)

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