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Proceedings Paper

Compact USB-powered mobile ELISA-based pathogen detection: design and implementation challenges
Author(s): Dmitry Starodubov; Anya Asanbaeva; Ihor Berezhnyy; Chung-Yen Chao; Richard Koziol; David Miller; Edward Patton; Sushma Trehan; Chris Ulmer
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Paper Abstract

Physical Optics Corporation (POC) presents a novel Mobile ELISA-based Pathogen Detection system that is based on a disposable microfluidic chip for multiple-threat detection and a highly sensitive portable microfluidic fluorescence measurement unit that also controls the flow of samples and reagents through the microfluidic channels of the chip. The fluorescence detection subsystem is composed of a commercial 635-nm diode laser, an avalanche photodiode (APD) that measures fluorescence, and three filtering mirrors that provide more than 100 dB of excitation line suppression in the signal detection channel. Special techniques to suppress the fluorescence and scattering background allow optimizing the dynamic range for a compact package. Concentrations below 100 ng/mL can be reliably identified. The entire instrument is powered using a USB port of a notebook PC and operates as a plug-and-play human-interface device, resulting in a truly peripheral biosensor. The operation of the system is fully automated, with minimal user intervention through the detection process. The resolved challenges of the design and implementation are presented in detail in this publication.

Paper Details

Date Published: 26 May 2011
PDF: 7 pages
Proc. SPIE 8024, Advanced Environmental, Chemical, and Biological Sensing Technologies VIII, 80240Z (26 May 2011); doi: 10.1117/12.884338
Show Author Affiliations
Dmitry Starodubov, Physical Optics Corp. (United States)
Anya Asanbaeva, Physical Optics Corp. (United States)
Ihor Berezhnyy, Physical Optics Corp. (United States)
Chung-Yen Chao, Physical Optics Corp. (United States)
Richard Koziol, Physical Optics Corp. (United States)
David Miller, Physical Optics Corp. (United States)
Edward Patton, Physical Optics Corp. (United States)
Sushma Trehan, Physical Optics Corp. (United States)
Chris Ulmer, Physical Optics Corp. (United States)

Published in SPIE Proceedings Vol. 8024:
Advanced Environmental, Chemical, and Biological Sensing Technologies VIII
Tuan Vo-Dinh; Robert A. Lieberman; Günter Gauglitz, Editor(s)

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