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Proceedings Paper

Dynamic staining of bacteria at a single-cell level
Author(s): Vicente Nuñez; Srigokul Upadhyayula; Adam Lin; Kenny Chau; Valentine I. Vullev
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Paper Abstract

Bacterial infectious diseases remain one of the major health hazards nation- and worldwide. The expedience of detection and identification of bacterial pathogens determines how early the diagnosis is, and hence, what the treatment and the outcome of the illness would be. As we have previously reported, the dynamics of fluorescence staining provides venues for the development of expedient assays for detection and identification of bacterial species[1]. We measured the kinetics of bacterial staining with cyanine and thioflavin dyes and investigated their photophysical properties. We demonstrated that the pseudo first-order kinetic constants of the fluorescence staining processes have species specificity without contrition dependence. Combining the dynamics of staining with real-time fluorescence microscopy we characterized the fluorescence staining process at the single-cell level with improved sensitivity and contrast.

Paper Details

Date Published: 3 June 2011
PDF: 7 pages
Proc. SPIE 8018, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XII, 80180A (3 June 2011); doi: 10.1117/12.884186
Show Author Affiliations
Vicente Nuñez, Univ. of California, Riverside (United States)
Srigokul Upadhyayula, Univ. of California, Riverside (United States)
Adam Lin, Univ. of California, Riverside (United States)
Kenny Chau, Univ. of California, Riverside (United States)
Valentine I. Vullev, Univ. of California, Riverside (United States)


Published in SPIE Proceedings Vol. 8018:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XII
Augustus W. Fountain; Patrick J. Gardner, Editor(s)

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