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Proceedings Paper

Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer
Author(s): Nan Bai; Yanji Tang; Arun K. Bhunia; E. Daniel Hirleman; Euiwon Bae
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Paper Abstract

To experimentally analyze the morphological characteristics and to predict the resulting scattering patterns of different bacterial colonies, an optical morphology analyzer was constructed based on a laser confocal displacement meter to simultaneously obtain the optical properties of colonies. The profile data was accurately captured using the confocal laser triangulation technology and the transmitted light was collected by a photodiode circuit. The analog signals were read into a data acquisition board in parallel for off-line signal processing. This approach showed promising results for differentiation of micro-colonies in the range of 100~300 μm based on the morphological differences among different species using light scattering.

Paper Details

Date Published: 12 May 2011
PDF: 8 pages
Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 80270C (12 May 2011); doi: 10.1117/12.884013
Show Author Affiliations
Nan Bai, Purdue Univ. (United States)
Yanji Tang, Purdue Univ. (United States)
Arun K. Bhunia, Purdue Univ. (United States)
E. Daniel Hirleman, Purdue Univ. (United States)
Univ. of California, Merced (United States)
Euiwon Bae, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 8027:
Sensing for Agriculture and Food Quality and Safety III
Moon S. Kim; Shu-I Tu; Kaunglin Chao, Editor(s)

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