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Proceedings Paper

Light without substrate amendment: the bacterial luciferase gene cassette as a mammalian bioreporter
Author(s): Dan M. Close; Tingting Xu; Abby E. Smartt; Pat Jegier; Steven A. Ripp; Gary S. Sayler
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Paper Abstract

Bioluminescent production represents a facile method for bioreporter detection in mammalian tissues. The lack of endogenous bioluminescent reactions in these tissues allows for high signal to noise ratios even at low signal strength compared to fluorescent signal detection. While the luciferase enzymes commonly employed for bioluminescent detection are those from class Insecta (firefly and click beetle luciferases), these are handicapped in that they require concurrent administration of a luciferin compound to elicit a bioluminescent signal. The bacterial luciferase (lux) gene cassette offers the advantages common to other bioluminescent proteins, but is simultaneously capable of synthesizing its own luciferin substrates using endogenously available cellular compounds. The longstanding shortcoming of the lux cassette has been its recalcitrance to function in the mammalian cellular environment. This paper will present an overview of the work completed to date to overcome this limitation and provide examples of mammalian lux-based bioreporter technologies that could provide the framework for advanced, biomedically relevant real-time sensor development.

Paper Details

Date Published: 16 May 2011
PDF: 10 pages
Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 80290F (16 May 2011); doi: 10.1117/12.883289
Show Author Affiliations
Dan M. Close, Univ. of Tennessee (United States)
Tingting Xu, Univ. of Tennessee (United States)
Abby E. Smartt, Univ. of Tennessee (United States)
Pat Jegier, Univ. of Tennessee (United States)
Steven A. Ripp, Univ. of Tennessee (United States)
Gary S. Sayler, Univ. of Tennessee (United States)


Published in SPIE Proceedings Vol. 8029:
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII
B. V. K. Vijaya Kumar; Salil Prabhakar; Arun A. Ross; Sárka O. Southern; Kevin N. Montgomery; Carl W. Taylor; Bernhard H. Weigl, Editor(s)

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