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Proceedings Paper

Instrument-free nucleic acid amplification assays for global health settings
Author(s): Paul LaBarre; David Boyle; Kenneth Hawkins; Bernhard Weigl
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Paper Abstract

Many infectious diseases that affect global health are most accurately diagnosed through nucleic acid amplification and detection. However, existing nucleic acid amplification tests are too expensive and complex for most low-resource settings. The small numbers of centralized laboratories that exist in developing countries tend to be in urban areas and primarily cater to the affluent. In contrast, rural area health care facilities commonly have only basic equipment and health workers have limited training and little ability to maintain equipment and handle reagents.1 Reliable electric power is a common infrastructure shortfall. In this paper, we discuss a practical approach to the design and development of non-instrumented molecular diagnostic tests that exploit the benefits of isothermal amplification strategies. We identify modular instrument-free technologies for sample collection, sample preparation, amplification, heating, and detection. By appropriately selecting and integrating these instrument-free modules, we envision development of an easy to use, infrastructure independent diagnostic test that will enable increased use of highly accurate molecular diagnostics at the point of care in low-resource settings.

Paper Details

Date Published: 16 May 2011
PDF: 15 pages
Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 802902 (16 May 2011); doi: 10.1117/12.882868
Show Author Affiliations
Paul LaBarre, PATH (United States)
David Boyle, PATH (United States)
Kenneth Hawkins, PATH (United States)
Bernhard Weigl, PATH (United States)


Published in SPIE Proceedings Vol. 8029:
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII
B. V. K. Vijaya Kumar; Sárka O. Southern; Kevin N. Montgomery; Salil Prabhakar; Arun A. Ross; Carl W. Taylor; Bernhard H. Weigl, Editor(s)

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