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Proceedings Paper

Raman spectroscopy for the characterization of algal cells
Author(s): Ota Samek; Alexandr Jonáš; Zdeněk Pilát; Pavel Zemánek; Ladislav Nedbal; Jan Tříska; Petr Kotas; Martin Trtílek
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Paper Abstract

Raman spectroscopy can elucidate fundamental questions about intercellular variability and what governs it. Moreover, knowing the metabolic response on single cell level this can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy is capable to measure nutrient dynamics and metabolism in vivo, in real-time, label free making it possible to monitor/evaluate population variability. Also, degree of unsaturation of the algae oil (iodine value) can be measured using Raman spectra obtained from single microalgae. The iodine value is the determination of the amount of unsaturation contained in fatty acids (in the form of double bonds). Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. We employed the characteristic peaks in the Raman scattering spectra at 1,656 cm-1 (cis C=C stretching mode) and 1,445 cm-1 (CH2 scissoring mode) as the markers defining the ratio of unsaturated-to-saturated carbon-carbon bonds of the fatty acids in the algal lipids.

Paper Details

Date Published: 15 December 2010
PDF: 7 pages
Proc. SPIE 7746, 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 77460X (15 December 2010); doi: 10.1117/12.882196
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Alexandr Jonáš, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Zdeněk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Ladislav Nedbal, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Jan Tříska, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Petr Kotas, Institute of Systems Biology and Ecology of the ASCR, v.v.i. (Czech Republic)
Martin Trtílek, Photon Systems Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 7746:
17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jarmila Müllerová; Dagmar Senderáková; Stanislav Jurečka, Editor(s)

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