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Proceedings Paper

Dynamical deformation compensation of phase in digital holographic microscopy
Author(s): Tomáš Zikmund; Lukáš Kvasnica; Hana Uhlířová; Luděk Lovicar; Radim Chmelík
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Paper Abstract

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Paper Details

Date Published: 15 December 2010
PDF: 7 pages
Proc. SPIE 7746, 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 77460K (15 December 2010); doi: 10.1117/12.882171
Show Author Affiliations
Tomáš Zikmund, Brno Univ. of Technology (Czech Republic)
Lukáš Kvasnica, Brno Univ. of Technology (Czech Republic)
Hana Uhlířová, Brno Univ. of Technology (Czech Republic)
Luděk Lovicar, Brno Univ. of Technology (Czech Republic)
Radim Chmelík, Brno Univ. of Technology (Czech Republic)


Published in SPIE Proceedings Vol. 7746:
17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Jarmila Müllerová; Dagmar Senderáková; Stanislav Jurečka, Editor(s)

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