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Proceedings Paper

A CARS solution with high temporal resolution
Author(s): Stefanie Landwehr; Vanessa Lurquin; William C. Hay; Vishnu Krishnamachari; Ulf Schwarz
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Paper Abstract

Confocal and multiphoton microscopy are powerful fluorescence techniques for morphological and dynamics studies of labeled elements. For non-fluorescent components, CARS (Coherent Anti-Stokes Raman Scattering) microscopy can be used for imaging various elements of cells such as lipids, proteins, DNA, etc. This technique is based on the intrinsic vibrational properties of the molecules. Leica Microsystems has combined CARS technology with its TCS SP5 II confocal microscope to provide several advantages for CARS imaging. The Leica TCS CARS combines two technologies in one system: a conventional scanner for maximum accuracy and a resonant scanner for highly time resolved imaging. For CARS microscopy, two picosecond near-infrared lasers are overlapped tightly, spatially and temporally, and sent directly into the confocal system. The conventional scanner can be used for morphological studies and the resonant scanner for following dynamic processes of unstained living cells. The fast scanner has several advantages over other solutions. First, the sectioning is truly confocal and does not suffer from spatial leakage. Second, the high speed (29 images/sec @ 512x512 pixels) provides fast data acquisition at video rates, allowing studies at the sub-cellular level. In summary, CARS microscopy combined with the tandem scanner makes the Leica TCS CARS a powerful tool for multimodal and three-dimensional imaging of chemical and biological sample.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031C (11 February 2011); doi: 10.1117/12.881932
Show Author Affiliations
Stefanie Landwehr, Leica Microsystems CMS GmbH (Germany)
Vanessa Lurquin, Leica Microsystems CMS GmbH (Germany)
William C. Hay, Leica Microsystems CMS GmbH (Germany)
Vishnu Krishnamachari, Leica Microsystems CMS GmbH (Germany)
Ulf Schwarz, Leica Microsystems CMS GmbH (Germany)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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