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Proceedings Paper

Femtosecond laser patterning of biological materials
Author(s): Costas P. Grigoropoulos; Hojeong Jeon; Hirofumi Hidai; David J. Hwang
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Paper Abstract

This paper aims at presenting a review of work at the Laser Thermal Laboratory on the microscopic laser modification of biological materials using ultrafast laser pulses. We have devised a new method for fabricating high aspect ratio patterns of varying height by using two-photon polymerization process in order to study contact guidance and directed growth of biological cells. Studies using NIH-3T3 and MDCK cells indicate that cell morphology on fiber scaffolds is influenced by the pattern of actin microfilament bundles. Cells experienced different strength of contact guidance depending on the ridge height. Cell morphology and motility was investigated on micronscale anisotropic cross patterns and parallel line patterns having different aspect ratios. A significant effect on cell alignment and directionality of migration was observed. Cell morphology and motility were influenced by the aspect ratio of the cross pattern, the grid size, and the ridge height. Cell contractility was examined microscopically in order to measure contractile forces generated by individual cells on self-standing fiber scaffolds.

Paper Details

Date Published: 11 February 2011
PDF: 9 pages
Proc. SPIE 7925, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI, 792506 (11 February 2011); doi: 10.1117/12.881707
Show Author Affiliations
Costas P. Grigoropoulos, Univ. of California, Berkeley (United States)
Hojeong Jeon, Univ. of California, Berkeley (United States)
Hirofumi Hidai, Univ. of California, Berkeley (United States)
David J. Hwang, Univ. of California, Berkeley (United States)


Published in SPIE Proceedings Vol. 7925:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI
Alexander Heisterkamp; Joseph Neev; Stefan Nolte, Editor(s)

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