Share Email Print
cover

Proceedings Paper

Rapid and field-deployable biological and chemical Raman-based identification
Author(s): Edita Botonjic-Sehic; Tracy L. Paxon; Hacene Boudries
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Pathogen detection using Raman spectroscopy is achieved through the use of a sandwich immunoassay. Antibody-modified magnetic beads are used to capture and concentrate target analytes in solution and surface-enhanced Raman spectroscopy (SERS) tags are conjugated with antibodies and act as labels to enable specific detection of biological pathogens. The rapid detection of biological pathogens is critical to first responders, thus assays to detect E.Coli and Anthrax have been developed and will be reported. The problems associated with pathogen detection resulting from the spectral complexity and variability of microorganisms are overcome through the use of SERS tags, which provide an intense, easily recognizable, and spectrally consistent Raman signal. The developed E. coli assay has been tested with 5 strains of E. coli and shows a low limit of detection, on the order of 10 and 100 c.f.u. per assay. Additionally, the SERS assay utilizes magnetic beads to collect the labeled pathogens into the focal point of the detection laser beam, making the assay robust to commonly encountered white powder interferants such as flour, baking powder, and corn starch. The reagents were also found to be stable at room temperature over extended periods of time with testing conducted over a one year period. Finally, through a specialized software algorithm, the assays are interfaced to the Raman instrument, StreetLab Mobile, for rapid-field-deployable biological identification.

Paper Details

Date Published: 12 May 2011
PDF: 9 pages
Proc. SPIE 8032, Next-Generation Spectroscopic Technologies IV, 80320G (12 May 2011); doi: 10.1117/12.881612
Show Author Affiliations
Edita Botonjic-Sehic, Morpho Detection (United States)
Tracy L. Paxon, GE Global Research (United States)
Hacene Boudries, Morpho Detection (United States)


Published in SPIE Proceedings Vol. 8032:
Next-Generation Spectroscopic Technologies IV
Mark A. Druy; Richard A. Crocombe, Editor(s)

© SPIE. Terms of Use
Back to Top